Table showing the results of the effect of the addition of lead nitrate and thiourea on urease activity
Boiling tube
pH in each boiling tube
Time(minute)
5
10
15
20
1
9.30
9.36
9.47
9.66
2
9.33
9.47
9.49
9.54
3
9.37
9.56
9.60
9.70
4
5.30
5.21
5.11
5.14
5
6.78
725
7.65
7.78
6
5.76
5.67
5.63
4.57
Graph showing the results of the effect of the addition of lead nitrate and thiourea on urease activity
Observations:
Before Urease was added pH was 5.3.When Urease was added to the first boiling tube it turned green after 5 minutes with a little fizzling at the top of the solution, also the pH in the first boiling tube increased as the time increased. When urease was added to the 2nd boiling tube it also turned green with after 5 minutes with no fizzling at the top of the top of the solution. As time increased so did the pH in the second boiling tube. When urease was added to the 3rd boiling tube it turned green within 5 minutes and also as the time increased with the urease inside the boiling tube its pH also increased with a little fizzling at the top of the solution. When urease was added to the 4th boiling tube it turned peachy orange and also as the urease was being check for its pH it decrease as the time was increased with a lot of fizzling in the boiling tube. When urease was added to the 5th boiling tube
Explanation:
A strong base was formed when urease was added to solution 1 and 2, for the urease catalyzed the reaction of urea and water whiles the buffer offered an acidic channel in order to get the reaction to respond. In solution 1 the urea and the distilled water has provided ammonia with the aid of the urease enzyme. In solution 1 and 2 we see that the solution becomes stronger as the time increases but solution 1 became stronger as the time was increased to 20 minutes this shows that the addition of thiourea brought the pH of the base produced a little and not too much so then the activity of the urease was not in anyway affected by thiourea because the urease still catalyzed the reaction of urea and distilled water. Solution 3 started out as a strong acid, the pH of solution 3 rose above solution 1 and 2 due to the presence of distilled water, which made the urease react properly with urea and the ammonia, which dissolved in water to produce a strong base. Solution 4 started out as a weak acid but the addition of urease neutralized it, the pH of 3 did not rise as much as 1 and 2 due to the absence of the distilled water in order for the urease to properly react with the urea and the ammonia to dissolve in the water and produce a strong base. The product of the reaction was also not able to properly stabilize to become a strong base due to the absence of water. Unlike the thiourea, lead nitrate had a major effect on the activity of urease, although 4th and 5th test turned peachy, they have different pH. The 6th test, which was the control, the 5th test was lower than it.
EVALUATION
I CONTEMPLATE THAT, OVERALL THE EXPERINMENT WAS FAIR. THERE MIGHT HAVE BEEN SOME MEASUREMENT ERRORS BUT IT WASN’T THAT SERIOUS IN ORDER TO MAKE THE EXPERINMENT ERRONEOUS AND UNREALIABLE.
